Browsing Kjemisk institutt by Author "Wilson, Steven Ray Haakon"

Now showing items 1-20 of 32

  • 3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry 
    Lin, Ann; Skottvoll, Frøydis Sved; Rayner, Simon; Pedersen-Bjergaard, Stig; Sullivan, Gareth; Krauss, Stefan; Wilson, Steven Ray Haakon; Harrison, Sean (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)
    In vitro derived simplified 3D representations of human organs or organ functionalities are predicted to play a major role in disease modeling, drug development, and personalized medicine, as they complement traditional ...
  • A global metabolomics minefield: Confounding effects of preanalytical factors when studying rare disorders 
    Skogvold, Hanne Bendiksen; Wilson, Steven Ray Haakon; Rønning, Per Ola; Ferrante, Linda; Opdal, Siri Hauge; Rognum, Torleiv Ole; Rootwelt, Helge; Elgstøen, Katja B. Prestø (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)
    Abstract A common challenge when studying rare diseases or medical conditions is the limited number of patients, usually resulting in long inclusion periods as well as unequal sampling and storage conditions. The main ...
  • Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes 
    Skogvold, Hanne Bendiksen; Sandås, Elise Mørk; Østeby, Anja; Løkken, Camilla; Rootwelt, Helge; Rønning, Per Ola; Wilson, Steven Ray Haakon; Elgstøen, Katja Benedikte Prestø (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
    Dried blood spot (DBS) metabolite analysis is a central tool for the clinic, e.g., newborn screening. Instead of applying multiple analytical methods, a single liquid chromatography-mass spectrometry (LC–MS) method was ...
  • Challenges for measuring oxytocin: The blind men and the elephant? 
    MacLean, Evan L.; Wilson, Steven Ray Haakon; Martin, W. Lance; Davis, John; Nazarloo, Hossein P.; Carter, C. Sue (Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2019)
    Since its discovery more than a century ago, oxytocin has become one of the most intensively studied molecules in behavioral biology. In the last five years, Psychoneuroendocrinology has published more than 500 articles ...
  • Combining HIC, SEC, and IEX with Fluorescence Polarization for Drug Target Discovery 
    Vehus, Tore; Waaler, Jo; Krauss, Stefan; Lundanes, Elsa; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)
    Fluorescence polarization (FP) is a highly regarded technique for studying drug–protein interactions, but has limited value regarding protein mixtures. As a novel approach to drug target discovery, the possibility of ...
  • Determination of perfluorooctane sulfonate and perfluorooctanoic acid in human plasma by packed capillary column switching coupled to micro-ESI-TOF-MS 
    Wilson, Steven Ray Haakon (Master thesis / Hovedoppgave, 2003)
    ABSTRACT The present work displays capillary liquid chromatographic column switching methodology tailored for fast, sensitive and selective determination of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid ...
  • Direct Electromembrane Extraction based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids 
    Wilson, Steven Ray Haakon; Krauss, Stefan Johannes Karl; Lundanes, Elsa; Skottvoll, Frøydis Sved; Bogen, Inger Lise; Pedersen-Bjergaard, Stig; Hansen, Frederik; Aizenshtadt, Aleksandra; Kutter, Jörg P.; Andersen, Jannike Mørch; Martinez, Mikel Amirola (Journal article / Tidsskriftartikkel / PublishedVersion, 2021)
    This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of ...
  • Distinguishing Between Cyclopropylfentanyl and Crotonylfentanyl by Methods Commonly Available in the Forensic Laboratory 
    Bergh, Marianne Skov-Skov; Bogen, Inger Lise; Wohlfarth, Ariane; Wilson, Steven Ray Haakon; Øiestad, Åse Marit Leere (Journal article / Tidsskriftartikkel / SubmittedVersion, 2019)
    Background: The opioid analgesic fentanyl and its analogues pose a major health concern due to its high potency and the increasing number of overdose deaths worldwide. The analogues of fentanyl may differ in potency, ...
  • Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry 
    Skogvold, Hanne Bendiksen; Rootwelt, Helge; Reubsaet, Leon; Elgstøen, Katja B. Prestø; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)
    Dried blood spot samples are simple to prepare and transport, enabling safe andaccessible diagnostics, both locally and globally. We review dried blood spot sam-ples for clinical analysis, focusing on liquid chromatography-mass ...
  • Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies 
    Skottvoll, Frøydis Sved; Hansen, Frederik; Harrison, Sean; Boger, Ida Caroline Sneis; Mrsa, Ago; Restan, Magnus Saed; Stein, Matthias; Lundanes, Elsa; Pedersen-Bjergaard, Stig; Aizenshtadt, Aleksandra; Krauss, Stefan; Sullivan, Gareth; Bogen, Inger Lise; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
    Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating ...
  • Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumour samples 
    Solheim, Stian; Hutchinson, Samantha A; Lundanes, Elsa; Wilson, Steven Ray Haakon; Thorne, James L; Røberg-Larsen, Hanne (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
    Oxysterols can contribute to proliferation of breast cancer through activation of the Estrogen Receptors, and to metastasis through activation of the Liver X Receptors. Endogenous levels of both esterified and free ...
  • Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols[S] 
    Røberg-Larsen, Hanne; Lund, Kaja; Vehus, Tore; Hoen, Nina; Vesterdal, Caroline; Misaghian, Dorna; Olsen, Peter Angell; Krauss, Stefan; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)
    Iso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method. ...
  • Integrated enzyme reactor and high resolving chromatography in ‘‘sub-chip’’ dimensions for sensitive protein mass spectrometry 
    Hustoft, Hanne Kolsrud; Brandtzæg, Ole Kristian Merkesvik; Misaghian, Dorna; Røgeberg, Magnus; Torsetnes, Silje Bøen; Reubsaet, Leon; Wilson, Steven Ray Haakon; Greibrokk, Tyge; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2013)
    Reliable, sensitive and automatable analytical methodology is of great value in e.g. cancer diagnostics. In this context, an on-line system for enzymatic cleavage of proteins, subsequent peptide separation by liquid ...
  • Is oxytocin “nature’s medicine”? 
    Sue Carter, Carter; Kenkel, William M.; MacLean, Evan L.; Wilson, Steven Ray Haakon; Perkeybile, Allison M.; Yee, Jason R.; Ferris, Craig F.; Nazarloo, Hossein P.; Porges, Stephen W.; Davis, John M.; Connelly, Jessica J.; Kingsbury, Marcy A. (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)
    Oxytocin is a pleiotropic, peptide hormone with broad implications for general health, adaptation, development, reproduction, and social behavior. Endogenous oxytocin and stimulation of the oxytocin receptor support patterns ...
  • Liquid chromatography-mass spectrometry platform for both small neurotransmitters and neuropeptides in blood, With Automatic and robust solid phase extraction 
    Johnsen, Elin Follaug; Lundanes, Elsa; Wilson, Steven Ray Haakon; Leknes, Siri (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)
    Neurons communicate via chemical signals called neurotransmitters (NTs). The numerous identified NTs can have very different physiochemical properties (solubility, charge, size etc.), so quantification of the various NT ...
  • Mass spectrometry reveals that oxysterols are secreted from non-alcoholic fatty liver disease induced organoids 
    Kømurcu, Kristina Sæterdal; Wilhelmsen, Ingrid; Thorne, James L.; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon; Aizenshtadt, Aleksandra; Røberg-Larsen, Hanne (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)
    Oxysterols are potential biomarkers for liver metabolism that are altered under disease conditions such as non-alcoholic fatty liver disease (NAFLD). We here apply sterolomics to organoids used for disease modeling of ...
  • Mass spectrometry-based measurements of cyclic adenosine monophosphate in cells, simplified using reversed phase liquid chromatography with a polar characterized stationary phase 
    Tsjokajev, Ahmad; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Anne Berit Dyve, Lingelem; Skotland, Tore; Sandvig, Kirsten; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)
    3′, 5′ – Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that is involved in many cellular functions and biological processes. In several cell types, cholera toxin will increase the level of cAMP, ...
  • Nano liquid chromatography columns 
    Wilson, Steven Ray Haakon; Olsen, Christine; Lundanes, Elsa (Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2019)
    Nano liquid chromatography (nanoLC), with columns having an inner diameter (ID) of ≤100 μm, can provide enhanced sensitivity and enable analysis of limited samples. NanoLC has become an established tool in omics research, ...
  • Open tubular lab-on-column/mass spectrometry for targeted proteomics of nanogram sample amounts 
    Hustoft, Hanne Kolsrud; Vehus, Tore; Brandtzæg, Ole Kristian Merkesvik; Krauss, Stefan; Greibrokk, Tyge; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)
    A novel open tubular nanoproteomic platform featuring accelerated on-line protein digestion and high-resolution nano liquid chromatography mass spectrometry (LC-MS) has been developed. The platform features very narrow ...
  • "Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry 
    Kogler, Stian; Aizenshtadt, Aleksandra; Harrison, Sean; Skottvoll, Frøydis Sved; Berg, Henriette Engen; Abadpour, Shadab; Scholz, Hanne; Sullivan, Gareth John; Thiede, Bernd; Lundanes, Elsa; Bogen, Inger Lise; Krauss, Stefan Johannes Karl; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
    Organoids, i.e., laboratory-grown organ models developed from stem cells, are emerging tools for studying organ physiology, disease modeling, and drug development. On-line analysis of organoids with mass spectrometry would ...