Browsing Kjemisk institutt by Author "Røberg-Larsen, Hanne"

Now showing items 1-15 of 15

  • Determination of oxysterols in cancer stem cells using on-line automated filtration and filter-flush solid phase extraction liquid chromatography tandem mass spectrometry 
    Røberg-Larsen, Hanne (Master thesis / Masteroppgave, 2011)
    A robust on-line automatic filtration and filter-flush solid phase extraction liquid chromatography tandem mass spectrometry (AFFL-SPE-LC- MS/MS) method for determination of 25hydroxycholesterol (25HC), 24S hydroxycholesterol ...
  • ER-Negative Breast Cancer Is Highly Responsive to Cholesterol Metabolite Signalling 
    Hutchinson, Samantha A; Lianto, Priscilia; Røberg-Larsen, Hanne; Battaglia, Sebastiano; Hughes, Thomas A; Thorne, James L (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
    Interventions that alter cholesterol have differential impacts on hormone receptor positive- and negative-breast cancer risk and prognosis. This implies differential regulation or response to cholesterol within different ...
  • Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumour samples 
    Solheim, Stian; Hutchinson, Samantha A; Lundanes, Elsa; Wilson, Steven Ray Haakon; Thorne, James L; Røberg-Larsen, Hanne (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
    Oxysterols can contribute to proliferation of breast cancer through activation of the Estrogen Receptors, and to metastasis through activation of the Liver X Receptors. Endogenous levels of both esterified and free ...
  • High sensitivity measurements of side-chain oxysterols using liquid chromatography mass spectrometry 
    Røberg-Larsen, Hanne (Doctoral thesis / Doktoravhandling, 2016)
  • Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols[S] 
    Røberg-Larsen, Hanne; Lund, Kaja; Vehus, Tore; Hoen, Nina; Vesterdal, Caroline; Misaghian, Dorna; Olsen, Peter Angell; Krauss, Stefan; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)
    Iso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method. ...
  • Impaired LXRα Phosphorylation Attenuates Progression of Fatty Liver Disease 
    Becares, Natalia; Cage, Matthew C; Voisin, Maud; Shrestha, Elina; Martin-Gutierrez, Lucia; Liang, Ning; Louie, Rikah; Pourcet, Benoit; Pello, Oscar M; Luong, Tu Vinh; Goni, Saioa; Pichardo-Almarza, Cesar; Røberg-Larsen, Hanne; Diaz-Zucccarini, Vanessa; Steffensen, Knut R.; O'Brien, Alastair; Garabedian, Michale J.; Rombouts, Krista; Treuter, Eckardt; Pineda-Torra, Ines (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
    Non-alcoholic fatty liver disease (NAFLD) is a very common indication for liver transplantation. How fat-rich diets promote progression from fatty liver to more damaging inflammatory and fibrotic stages is poorly understood. ...
  • Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer 
    Hutchinson, Samantha A.; Websdale, Alex; Cioccoloni, Giorgia; Røberg-Larsen, Hanne; Lianto, Priscilia; Kim, Baek; Rose, Ailsa; Soteriou, Chrysa; Pramanik, Arindam; Wastall, Laura M.; Williams, Bethany J.; Henn, Madeline A.; Chen, Joy J.; Ma, Liqian; Moore, J. Bernadette; Nelson, Erik; Hughes, Thomas A.; Thorne, James L. (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
    Abstract Triple negative breast cancer (TNBC) is challenging to treat successfully because targeted therapies do not exist. Instead, systemic therapy is typically restricted to cytotoxic chemotherapy, which fails more often ...
  • Mass spectrometry reveals that oxysterols are secreted from non-alcoholic fatty liver disease induced organoids 
    Kømurcu, Kristina Sæterdal; Wilhelmsen, Ingrid; Thorne, James L.; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon; Aizenshtadt, Aleksandra; Røberg-Larsen, Hanne (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)
    Oxysterols are potential biomarkers for liver metabolism that are altered under disease conditions such as non-alcoholic fatty liver disease (NAFLD). We here apply sterolomics to organoids used for disease modeling of ...
  • Mass spectrometry-based measurements of cyclic adenosine monophosphate in cells, simplified using reversed phase liquid chromatography with a polar characterized stationary phase 
    Tsjokajev, Ahmad; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Anne Berit Dyve, Lingelem; Skotland, Tore; Sandvig, Kirsten; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)
    3′, 5′ – Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that is involved in many cellular functions and biological processes. In several cell types, cholera toxin will increase the level of cAMP, ...
  • "Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry 
    Kogler, Stian; Aizenshtadt, Aleksandra; Harrison, Sean; Skottvoll, Frøydis Sved; Berg, Henriette Engen; Abadpour, Shadab; Scholz, Hanne; Sullivan, Gareth John; Thiede, Bernd; Lundanes, Elsa; Bogen, Inger Lise; Krauss, Stefan Johannes Karl; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
    Organoids, i.e., laboratory-grown organ models developed from stem cells, are emerging tools for studying organ physiology, disease modeling, and drug development. On-line analysis of organoids with mass spectrometry would ...
  • Organoids, organ-on-a-chip, separation science and mass spectrometry: An update 
    Kogler, Stian; Kømurcu, Kristina Sæterdal; Olsen, Christine Sandsnes; Shoji, Jun-ya; Skottvoll, Frøydis Sved; Krauss, Stefan Johannes Karl; Wilson, Steven Ray Haakon; Røberg-Larsen, Hanne (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)
    Organoids are 3D models of organs, grown in the laboratory from stem cells. An organ model grown/placed in microfluidic devices is commonly termed an “organ-on-a-chip”. Organoids and organ-on-a-chip devices are becoming ...
  • Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum 
    Brandtzaeg, Ole Kristian; Johnsen, Elin Follaug; Røberg-Larsen, Hanne; Seip, Knut Fredrik; MacLean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)
    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe ...
  • Rugged Large Volume Injection for Sensitive Capillary LC-MS Environmental Monitoring 
    Røberg-Larsen, Hanne; Abele, Silvija; Demir, Deniz; Dzabijeva, Diana; Amundsen, Sunniva Furre; Wilson, Steven Ray Haakon; Bartkevics, Vadims; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)
    A rugged and high throughput capillary column (cLC) LC-MS switching platform using large volume injection and on-line automatic filtration and filter back-flush (AFFL) solid phase extraction (SPE) for analysis of environmental ...
  • Ultracentrifugation versus kit exosome isolation: nanoLC–MS and other tools reveal similar performance biomarkers, but also contaminations 
    Skottvoll, Frøydis Sved; Berg, Henriette Engen; Bjørseth, Kamilla; Lund, Kaja; Roos, Norbert; Bekhradnia, Sara; Thiede, Bernd; Sandberg, Cecilie; Vik-Mo, Einar O.; Røberg-Larsen, Hanne; Nyström, Bo; Lundanes, Elsa; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2018)
    Aim: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. Materials & methods: ‘Case study’ exosomes isolated from patient-derived cells from glioblastoma ...
  • Versatile, sensitive liquid chromatography mass spectrometry–Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins 
    Vehus, Tore; Røberg-Larsen, Hanne; Waaler, Jo; Aslaksen, Sigrid; Krauss, Stefan; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)