Browsing Kjemisk institutt by Author "Lundanes, Elsa"
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Vehus, Tore; Waaler, Jo; Krauss, Stefan; Lundanes, Elsa; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)Fluorescence polarization (FP) is a highly regarded technique for studying drug–protein interactions, but has limited value regarding protein mixtures. As a novel approach to drug target discovery, the possibility of ...
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Wilson, Steven Ray Haakon; Krauss, Stefan Johannes Karl; Lundanes, Elsa; Skottvoll, Frøydis Sved; Bogen, Inger Lise; Pedersen-Bjergaard, Stig; Hansen, Frederik; Aizenshtadt, Aleksandra; Kutter, Jörg P.; Andersen, Jannike Mørch; Martinez, Mikel Amirola (Journal article / Tidsskriftartikkel / PublishedVersion, 2021)This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of ...
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Skottvoll, Frøydis Sved; Hansen, Frederik; Harrison, Sean; Boger, Ida Caroline Sneis; Mrsa, Ago; Restan, Magnus Saed; Stein, Matthias; Lundanes, Elsa; Pedersen-Bjergaard, Stig; Aizenshtadt, Aleksandra; Krauss, Stefan; Sullivan, Gareth; Bogen, Inger Lise; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating ...
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Solheim, Stian; Hutchinson, Samantha A; Lundanes, Elsa; Wilson, Steven Ray Haakon; Thorne, James L; Røberg-Larsen, Hanne (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)Oxysterols can contribute to proliferation of breast cancer through activation of the Estrogen Receptors, and to metastasis through activation of the Liver X Receptors. Endogenous levels of both esterified and free ...
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Poothong, Somrutai; Lundanes, Elsa; Thomsen, Cathrine; Haug, Line Småstuen (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)A rapid, sensitive and reliable method was developed for the determination of a broad range of poly- and perfluoroalkyl substances (PFASs) in various blood matrices (serum, plasma, and whole blood), and uses only 50 μL of ...
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Røberg-Larsen, Hanne; Lund, Kaja; Vehus, Tore; Hoen, Nina; Vesterdal, Caroline; Misaghian, Dorna; Olsen, Peter Angell; Krauss, Stefan; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)Iso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method. ...
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Hustoft, Hanne Kolsrud; Brandtzæg, Ole Kristian Merkesvik; Misaghian, Dorna; Røgeberg, Magnus; Torsetnes, Silje Bøen; Reubsaet, Leon; Wilson, Steven Ray Haakon; Greibrokk, Tyge; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2013)Reliable, sensitive and automatable analytical methodology is of great value in e.g. cancer diagnostics. In this context, an on-line system for enzymatic cleavage of proteins, subsequent peptide separation by liquid ...
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Johnsen, Elin Follaug; Lundanes, Elsa; Wilson, Steven Ray Haakon; Leknes, Siri (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2015)Neurons communicate via chemical signals called neurotransmitters (NTs). The numerous identified NTs can have very different physiochemical properties (solubility, charge, size etc.), so quantification of the various NT ...
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Tsjokajev, Ahmad; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon; Anne Berit Dyve, Lingelem; Skotland, Tore; Sandvig, Kirsten; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2020)3′, 5′ – Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that is involved in many cellular functions and biological processes. In several cell types, cholera toxin will increase the level of cAMP, ...
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Wilson, Steven Ray Haakon; Olsen, Christine; Lundanes, Elsa (Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2019)Nano liquid chromatography (nanoLC), with columns having an inner diameter (ID) of ≤100 μm, can provide enhanced sensitivity and enable analysis of limited samples. NanoLC has become an established tool in omics research, ...
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Hustoft, Hanne Kolsrud; Vehus, Tore; Brandtzæg, Ole Kristian Merkesvik; Krauss, Stefan; Greibrokk, Tyge; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)A novel open tubular nanoproteomic platform featuring accelerated on-line protein digestion and high-resolution nano liquid chromatography mass spectrometry (LC-MS) has been developed. The platform features very narrow ...
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Kogler, Stian; Aizenshtadt, Aleksandra; Harrison, Sean; Skottvoll, Frøydis Sved; Berg, Henriette Engen; Abadpour, Shadab; Scholz, Hanne; Sullivan, Gareth John; Thiede, Bernd; Lundanes, Elsa; Bogen, Inger Lise; Krauss, Stefan Johannes Karl; Røberg-Larsen, Hanne; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)Organoids, i.e., laboratory-grown organ models developed from stem cells, are emerging tools for studying organ physiology, disease modeling, and drug development. On-line analysis of organoids with mass spectrometry would ...
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Brandtzaeg, Ole Kristian; Johnsen, Elin Follaug; Røberg-Larsen, Hanne; Seip, Knut Fredrik; MacLean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe ...
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Røberg-Larsen, Hanne; Abele, Silvija; Demir, Deniz; Dzabijeva, Diana; Amundsen, Sunniva Furre; Wilson, Steven Ray Haakon; Bartkevics, Vadims; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2017)A rugged and high throughput capillary column (cLC) LC-MS switching platform using large volume injection and on-line automatic filtration and filter back-flush (AFFL) solid phase extraction (SPE) for analysis of environmental ...
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Zawadzka, Malgorzata Elzbieta; Racz, Beatrix; Ambrosini, Dario; Görbitz, Carl Henrik; Morth, Jens Preben; Wilkins, Alistair L.; Østeby, Anja; Elgstøen, Katja B. Prestø; Lundanes, Elsa; Rise, Frode; Ringvold, Amund; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)Abstract The eye lens is a unique organ as no cells can be replaced throughout life. This makes it decisive that the lens is protected against damaging UV-radiation. An ultraviolet (UV)-absorbing compound of unknown identity ...
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Olsen, Christine; Wang, Chencheng; Aizenshtadt, Aleksandra; Abadpour, Shadab; Lundanes, Elsa; Skottvoll, Frøydis Sved; Golovin, Alexey; Busek, Mathias; Krauss, Stefan Johannes Karl; Scholz, Hanne; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2023)Abstract For studying stem cell–derived islet organoids (SC‐islets) in an organ‐on‐chip (OoC) platform, we have developed a reversed‐phase liquid chromatography–tandem mass spectrometry (RPLC–MS/MS) method allowing for ...
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Ambaw, Yohannes Abere; Dahl, Sandra Rinne; Chen, Yan; Greibrokk, Tyge; Lundanes, Elsa; Lazraq, Issam; Shinde, Sudhirkumar; Selvalatchmanan, Jayashree; Wenk, Markus R.; Sellergren, Börje; Torta, Federico (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)Lipid mediators, small molecules involved in regulating inflammation and its resolution, are a class of lipids of wide interest as their levels in blood and tissues may be used to monitor health and disease states or the ...
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Røen, Bent Tore; Sellevåg, Stig Rune; Dybendal, Kjersti; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2014)A method for determination of the primary nerve agent degradation products ethyl-, isopropyl-, isobutyl-, cyclohexyl- and pinacolyl methylphosphonic acid in aqueous soil extracts has been developed utilizing on-line solid ...
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Skottvoll, Frøydis Sved; Berg, Henriette Engen; Bjørseth, Kamilla; Lund, Kaja; Roos, Norbert; Bekhradnia, Sara; Thiede, Bernd; Sandberg, Cecilie; Vik-Mo, Einar O.; Røberg-Larsen, Hanne; Nyström, Bo; Lundanes, Elsa; Wilson, Steven Ray Haakon (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2018)Aim: For isolation of exosomes, differential ultracentrifugation and an isolation kit from a major vendor were compared. Materials & methods: ‘Case study’ exosomes isolated from patient-derived cells from glioblastoma ...
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Vehus, Tore; Røberg-Larsen, Hanne; Waaler, Jo; Aslaksen, Sigrid; Krauss, Stefan; Wilson, Steven Ray Haakon; Lundanes, Elsa (Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2016)