dc.date.accessioned | 2015-03-26T14:48:27Z | |
dc.date.available | 2015-03-26T14:48:27Z | |
dc.date.created | 2014-11-10T14:49:08Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Edin, Nina Frederike J . The role of interleukin-13 in the removal of hyper-radiosensitivity by priming irradiation. Journal of radiation research. 2014 | |
dc.identifier.uri | http://hdl.handle.net/10852/43500 | |
dc.description.abstract | It has previously been demonstrated that the presence of fetal bovine serum is necessary for TGF-β3 (transforming growth factor beta 3)-dependent elimination of low-dose hyper-radiosensitivity (HRS) in cells by 1 h of low-dose-rate γ-irradiation (0.2–0.3 Gy/h). The purpose of the present study was to identify the serum constituent involved. Two human HRS-positive (T-47D, T98G) cell lines were used. The effects of different pretreatments on HRS were investigated using the colony assay. Total inducible nitric oxide synthase (iNOS) levels were measured using a cell-based ELISA assay. The serum factor was identified as interleukin-13 (IL-13). In order for low dose-rate irradiation to eliminate HRS through the TGF-β3-dependent mechanism, the cells must be exposed to IL-13 first. Inhibiting receptor IL-13Rα2 showed that this receptor is involved in the response. Adding IL-13 to serum-free medium restored the properties of full medium but not when an inhibitor of proprotein convertase activity was added together with IL-13. The presence of IL-13 resulted in upregulation of total iNOS protein levels. Thus, this study indicates that IL-13 interacts with the cells though receptor IL-13Rα2 and induces upregulation of iNOS and activation of one or more furin-like proprotein convertases. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | The role of interleukin-13 in the removal of hyper-radiosensitivity by priming irradiation | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Edin, Nina Frederike J | |
cristin.unitcode | 185,15,4,50 | |
cristin.unitname | Biofysikk og medisinsk fysikk | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1171631 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of radiation research&rft.volume=&rft.spage=&rft.date=2014 | |
dc.identifier.jtitle | Journal of radiation research | |
dc.identifier.volume | 55 | |
dc.identifier.issue | 6 | |
dc.identifier.startpage | 1066 | |
dc.identifier.endpage | 1074 | |
dc.identifier.doi | http://dx.doi.org/10.1093/jrr/rru053 | |
dc.identifier.urn | URN:NBN:no-47871 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0449-3060 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/43500/2/1066-full-edin.pdf | |
dc.type.version | PublishedVersion | |