dc.date.accessioned | 2015-03-18T15:16:57Z | |
dc.date.available | 2015-03-18T15:16:57Z | |
dc.date.created | 2014-09-19T11:32:29Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Khuu, Cuong Jevnaker, Anne Marthe Bryne, Magne Osmundsen, Harald . An investigation into anti-proliferative effects of microRNAs encoded by the miR-106a-363 cluster on human carcinoma cells and keratinocytes using microarray profiling of miRNA transcriptomes. Frontiers in Genetics. 2014, 5 | |
dc.identifier.uri | http://hdl.handle.net/10852/43284 | |
dc.description.abstract | Transfection of human oral squamous carcinoma cells (clone E10) with mimics for unexpressed miR-20b or miR-363-5p, encoded by the miR-106a-363 cluster (miR-20b, miR-106a, miR-363-3p, or miR-363-5p), caused 40–50% decrease in proliferation. Transfection with mimics for miR-18a or miR-92a, encoded by the miR-17-92 cluster (all members being expressed in E10 cells), had no effect on proliferation. In contrast, mimic for the sibling miRNA-19a yielded about 20% inhibition of proliferation. To investigate miRNA involvement profiling of miRNA transcriptomes were carried out using deoxyoligonucleotide microarrays. In transfectants for miR-19a, or miR-20b or miR-363-5p most differentially expressed miRNAs exhibited decreased expression, including some miRNAs encoded in paralogous miR-17-92—or miR-106b-25 cluster. Only in cells transfected with miR-19a mimic significantly increased expression of miR-20b observed—about 50-fold as judged by qRT-PCR. Further studies using qRT-PCR showed that transfection of E10 cells with mimic for miRNAs encoded by miR-17-92 - or miR-106a-363 - or the miR-106b-25 cluster confirmed selective effect on expression on sibling miRNAs. We conclude that high levels of miRNAs encoded by the miR-106a-363 cluster may contribute to inhibition of proliferation by decreasing expression of several sibling miRNAs encoded by miR-17-92 or by the miR-106b-25 cluster. The inhibition of proliferation observed in miR-19a-mimic transfectants is likely caused by the miR-19a-dependent increase in the levels of miR-20b and miR-106a. Bioinformatic analysis of differentially expressed miRNAs from miR-106a, miR-20b and miR-363-5p transfectants, but not miR-92a transfectants, yielded significant associations to “Cellular Growth and Proliferation” and “Cell Cycle.” Western blotting results showed that levels of affected proteins to differ between transfectants, suggesting that different anti-proliferative mechanisms may operate in these transfectants. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.rights | Attribution 3.0 Unported | |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
dc.title | An investigation into anti-proliferative effects of microRNAs encoded by the miR-106a-363 cluster on human carcinoma cells and keratinocytes using microarray profiling of miRNA transcriptomes | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Khuu, Cuong | |
dc.creator.author | Jevnaker, Anne Marthe | |
dc.creator.author | Bryne, Magne | |
dc.creator.author | Osmundsen, Harald | |
cristin.unitcode | 185,16,15,0 | |
cristin.unitname | Institutt for oral biologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1155981 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers in Genetics&rft.volume=5&rft.spage=&rft.date=2014 | |
dc.identifier.jtitle | Frontiers in Genetics | |
dc.identifier.volume | 5 | |
dc.identifier.doi | http://dx.doi.org/10.3389/fgene.2014.00246 | |
dc.identifier.urn | URN:NBN:no-47668 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1664-8021 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/43284/1/fgene-05-00246-khuu.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 246 | |