dc.date.accessioned | 2014-01-07T13:17:01Z | |
dc.date.available | 2014-01-07T13:17:01Z | |
dc.date.created | 2013-12-20T11:15:01Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Hustoft, Hanne Kolsrud Brandtzæg, Ole Kristian Merkesvik Misaghian, Dorna Røgeberg, Magnus Torsetnes, Silje Bøen Reubsaet, Leon Wilson, Steven Ray Haakon Greibrokk, Tyge Lundanes, Elsa . Integrated enzyme reactor and high resolving chromatography in ‘‘sub-chip’’ dimensions for sensitive protein mass spectrometry. Scientific Reports. 2013, 3 | |
dc.identifier.uri | http://hdl.handle.net/10852/37947 | |
dc.description.abstract | Reliable, sensitive and automatable analytical methodology is of great value in e.g. cancer diagnostics. In this context, an on-line system for enzymatic cleavage of proteins, subsequent peptide separation by liquid chromatography (LC) with mass spectrometric detection has been developed using “sub-chip” columns (10–20 µm inner diameter, ID). The system could detect attomole amounts of isolated cancer biomarker progastrin-releasing peptide (ProGRP), in a more automatable fashion compared to previous methods. The workflow combines protein digestion using an 20 µm ID immobilized trypsin reactor with a polymeric layer of 2-hydroxyethyl methacrylate-vinyl azlactone (HEMA-VDM), desalting on a polystyrene-divinylbenzene (PS-DVB) monolithic trap column, and subsequent separation of resulting peptides on a 10 µm ID (PS-DVB) porous layer open tubular (PLOT) column. The high resolution of the PLOT columns was maintained in the on-line system, resulting in narrow chromatographic peaks of 3–5 seconds. The trypsin reactors provided repeatable performance and were compatible with long-term storage. | |
dc.language | MU | |
dc.rights | Attribution 3.0 Unported | |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
dc.title | Integrated enzyme reactor and high resolving chromatography in ‘‘sub-chip’’ dimensions for sensitive protein mass spectrometry | |
dc.type | Journal article | |
dc.creator.author | Hustoft, Hanne Kolsrud | |
dc.creator.author | Brandtzæg, Ole Kristian Merkesvik | |
dc.creator.author | Misaghian, Dorna | |
dc.creator.author | Røgeberg, Magnus | |
dc.creator.author | Torsetnes, Silje Bøen | |
dc.creator.author | Reubsaet, Leon | |
dc.creator.author | Wilson, Steven Ray Haakon | |
dc.creator.author | Greibrokk, Tyge | |
dc.creator.author | Lundanes, Elsa | |
cristin.unitcode | 185,15,0,0 | |
cristin.unitname | Det matematisk-naturvitenskapelige fakultet | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1079957 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=3&rft.spage=&rft.date=2013 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 3 | |
dc.identifier.pagecount | 7 | |
dc.identifier.doi | http://dx.doi.org/10.1038/srep03511 | |
dc.identifier.urn | URN:NBN:no-40228 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/37947/1/Publisert_hustoft_2013.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 3511 | |