dc.date.accessioned | 2013-12-19T15:55:42Z | |
dc.date.available | 2013-12-19T15:55:42Z | |
dc.date.created | 2013-09-18T09:24:48Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Backe, Paul Hoff Simm, Roger Lærdahl, Jon Kristen Dalhus, Bjørn Fagerlund, Annette Økstad, Ole Andreas Løchen Rognes, Torbjørn Alseth, Ingrun Kolstø, Anne-Brit Bjørås, Magnar . A new family of proteins related to the HEAT-like repeat DNA glycosylases with affinity for branched DNA structures. Journal of Structural Biology. 2013, 183(1), 66-75 | |
dc.identifier.uri | http://hdl.handle.net/10852/37935 | |
dc.description.abstract | The recently discovered HEAT-like repeat (HLR) DNA glycosylase superfamily is widely distributed in all domains of life. The present bioinformatics and phylogenetic analysis shows that HLR DNA glycosylase superfamily members in the genus Bacillus form three subfamilies: AlkC, AlkD and AlkF/AlkG. The crystal structure of AlkF shows structural similarity with the DNA glycosylases AlkC and AlkD, however neither AlkF nor AlkG display any DNA glycosylase activity. Instead, both proteins have affinity to branched DNA structures such as three-way and Holliday junctions. A unique ß-hairpin in the AlkF/AlkG subfamily is most likely inserted into the DNA major groove, and could be a structural determinant regulating DNA substrate affinity. We conclude that AlkF and AlkG represent a new family of HLR proteins with affinity for branched DNA structures. | |
dc.language | EN | |
dc.publisher | Academic Press | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Unported | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.title | A new family of proteins related to the HEAT-like repeat DNA glycosylases with affinity for branched DNA structures | |
dc.type | Journal article | |
dc.creator.author | Backe, Paul Hoff | |
dc.creator.author | Simm, Roger | |
dc.creator.author | Lærdahl, Jon Kristen | |
dc.creator.author | Dalhus, Bjørn | |
dc.creator.author | Fagerlund, Annette | |
dc.creator.author | Økstad, Ole Andreas Løchen | |
dc.creator.author | Rognes, Torbjørn | |
dc.creator.author | Alseth, Ingrun | |
dc.creator.author | Kolstø, Anne-Brit | |
dc.creator.author | Bjørås, Magnar | |
cristin.unitcode | 185,53,18,14 | |
cristin.unitname | Avdeling for medisinsk biokjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1050122 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Structural Biology&rft.volume=183&rft.spage=66&rft.date=2013 | |
dc.identifier.jtitle | Journal of Structural Biology | |
dc.identifier.volume | 183 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 66 | |
dc.identifier.endpage | 75 | |
dc.identifier.doi | http://dx.doi.org/10.1016/j.jsb.2013.04.007 | |
dc.identifier.urn | URN:NBN:no-40031 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1047-8477 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/37935/1/1-s2.0-S104784771300107X-main.pdf | |
dc.type.version | PublishedVersion | |