Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates.
dc.date.accessioned | 2013-03-12T12:29:47Z | |
dc.date.available | 2013-03-12T12:29:47Z | |
dc.date.issued | 2008 | en_US |
dc.date.submitted | 2008-10-23 | en_US |
dc.identifier.citation | Avellan, Cecilia I. A.. Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates.. Prosjektoppgave, University of Oslo, 2008 | en_US |
dc.identifier.uri | http://hdl.handle.net/10852/28895 | |
dc.description.abstract | Cyclic AMP-dependent protein kinase (PKA) is a holoenzyme composed of two catalytic (C) subunits and a regulatory (R) subunit dimer. The holoenzyme dissociates when four cAMP molecules bind to the R subunit dimer. This activates the C subunits, allowing them to phosphorylate specific substrate proteins and thereby alter their activity. Studies involving cDNA cloning have revealed four different R and four different C subunit genes which encode the subunits designated RI, RI, RII, RII, C, C, C and PRKX1. Furthermore, different splice variants encoded by the human C and C genes have been identified and designated Cα1, CαS, Cβ1, Cβ2, C3, Cβ3b, Cβ3ab, Cβ3abc, C4, Cβ4b, C4ab and C4abc. They are all tissue-specifically expressed, highly heterogenic in the N-terminal end, and highly conserved in the catalytic domain. We have discovered 6 novel splice variants of the C gene in NTera2-N cells as both mRNAs and proteins. All the novel splice variants were designated CβΔ4 since they lack 99 encoding base pairs representing exon 4 due to inframe skipping in the C gene. We developed a method to detect CβΔ4 mRNAs and found that these are primarily expressed in the brain of higher primates. While separately co-expressing different CβΔ4 variants with either the RI or RII subunit, we demonstrated that CβΔ4 interacts with the R subunit, in a cAMP insensitive fashion. This together with the fact that these variants are brain specifically expressed may suggest that they function to regulate cAMP sensitivity of nerve cells. | eng |
dc.language.iso | eng | en_US |
dc.subject | ernæringslære | |
dc.title | Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates. | en_US |
dc.type | Master thesis | en_US |
dc.date.updated | 2009-01-21 | en_US |
dc.creator.author | Avellan, Cecilia I. A. | en_US |
dc.subject.nsi | VDP::811 | en_US |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Avellan, Cecilia I. A.&rft.title=Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates.&rft.inst=University of Oslo&rft.date=2008&rft.degree=Prosjektoppgave | en_US |
dc.identifier.urn | URN:NBN:no-20707 | en_US |
dc.type.document | Prosjektoppgave | en_US |
dc.identifier.duo | 86159 | en_US |
dc.contributor.supervisor | Bjørn Steen Skålhegg | en_US |
dc.identifier.bibsys | 090139380 | en_US |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/28895/2/Prosjektxavellan.pdf |
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