dc.date.accessioned | 2013-03-12T08:38:54Z | |
dc.date.issued | 2007 | en_US |
dc.date.submitted | 2007-08-16 | en_US |
dc.identifier.citation | Bjørnstad, Linn. Characterization of the human AlkB homologue 4 (hABH4). Masteroppgave, University of Oslo, 2007 | en_US |
dc.identifier.uri | http://hdl.handle.net/10852/11522 | |
dc.description.abstract | The E. coli AlkB protein reverses methylation damage from DNA and RNA by oxidative demethylation, a reaction dependent on Fe(II) and 2-oxoglutarate. Based on sequence similarity, eight human AlkB homologues, denoted hABH1-8, have been identified. hABH2 and hABH3 possess repair activities similar to that of AlkB. While hABH2 is preferentially active on double-stranded DNA substrates, hABH3 is also active on RNA and prefers singlestranded substrates. The functions of hABH1 and hABH4-8 are not yet discovered. These proteins have been suggested to function in regulation of gene expression, by demethylation of 5-methylcytosine on DNA or methylated lysine or arginine residues on histones. Here, the function of hABH4 has been investigated. The enzymatic activity of hABH4 towards 5-methylcytosine was tested in vitro and in vivo. No hABH4-mediated demethylation was observed. Two proteins identified as interactants of hABH4 in by the Yeast Two-Hybrid method, may indicate a role of hABH4 transcriptional regulation. GST pull-down studies were performed to verify these interactions, but a conclusion could not be made because of high background. Further study is needed for determination of the function of hABH4. | nor |
dc.language.iso | eng | en_US |
dc.subject | hABH4 metylering epigenetiske modifikasjoner | en_US |
dc.title | Characterization of the human AlkB homologue 4 (hABH4) | en_US |
dc.type | Master thesis | en_US |
dc.date.updated | 2007-09-26 | en_US |
dc.creator.author | Bjørnstad, Linn | en_US |
dc.subject.nsi | VDP::473 | en_US |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft.au=Bjørnstad, Linn&rft.title=Characterization of the human AlkB homologue 4 (hABH4)&rft.inst=University of Oslo&rft.date=2007&rft.degree=Masteroppgave | en_US |
dc.identifier.urn | URN:NBN:no-16053 | en_US |
dc.type.document | Masteroppgave | en_US |
dc.identifier.duo | 64408 | en_US |
dc.contributor.supervisor | Pål Falnes | en_US |
dc.identifier.bibsys | 070946175 | en_US |