dc.date.accessioned | 2024-04-03T15:58:19Z | |
dc.date.available | 2024-04-03T15:58:19Z | |
dc.date.created | 2024-01-08T19:50:06Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Nagpal, Harsh Ali Ahmad, Ahmad Hirano, Yasuhiro Cai, Wei Halic, Mario Fukagawa, Tatsuo Sekulic, Nikolina Fierz, Beat . CENP-A and CENP-B collaborate to create an open centromeric chromatin state. Nature Communications. 2023, 14(1) | |
dc.identifier.uri | http://hdl.handle.net/10852/110295 | |
dc.description.abstract | Centromeres are epigenetically defined via the presence of the histone H3 variant CENP-A. Contacting CENP-A nucleosomes, the constitutive centromere associated network (CCAN) and the kinetochore assemble, connecting the centromere to spindle microtubules during cell division. The DNA-binding centromeric protein CENP-B is involved in maintaining centromere stability and, together with CENP-A, shapes the centromeric chromatin state. The nanoscale organization of centromeric chromatin is not well understood. Here, we use single-molecule fluorescence and cryoelectron microscopy (cryoEM) to show that CENP-A incorporation establishes a dynamic and open chromatin state. The increased dynamics of CENP-A chromatin create an opening for CENP-B DNA access. In turn, bound CENP-B further opens the chromatin fiber structure and induces nucleosomal DNA unwrapping. Finally, removal of CENP-A increases CENP-B mobility in cells. Together, our studies show that the two centromere-specific proteins collaborate to reshape chromatin structure, enabling the binding of centromeric factors and establishing a centromeric chromatin state. | |
dc.description.abstract | CENP-A and CENP-B collaborate to create an open centromeric chromatin state | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | CENP-A and CENP-B collaborate to create an open centromeric chromatin state | |
dc.title.alternative | ENEngelskEnglishCENP-A and CENP-B collaborate to create an open centromeric chromatin state | |
dc.type | Journal article | |
dc.creator.author | Nagpal, Harsh | |
dc.creator.author | Ali Ahmad, Ahmad | |
dc.creator.author | Hirano, Yasuhiro | |
dc.creator.author | Cai, Wei | |
dc.creator.author | Halic, Mario | |
dc.creator.author | Fukagawa, Tatsuo | |
dc.creator.author | Sekulic, Nikolina | |
dc.creator.author | Fierz, Beat | |
cristin.unitcode | 185,57,0,0 | |
cristin.unitname | Norsk Senter for Molekylærmedisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 2222689 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature Communications&rft.volume=14&rft.spage=&rft.date=2023 | |
dc.identifier.jtitle | Nature Communications | |
dc.identifier.volume | 14 | |
dc.identifier.issue | 1 | |
dc.identifier.pagecount | 0 | |
dc.identifier.doi | https://doi.org/10.1038/s41467-023-43739-5 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2041-1723 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 8227 | |
dc.relation.project | NFR/325528 | |
dc.relation.project | NFR/187615 | |