dc.date.accessioned | 2024-03-15T18:05:03Z | |
dc.date.available | 2024-03-15T18:05:03Z | |
dc.date.created | 2023-02-26T15:17:53Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Grødem, Sverre Nymoen, Ingeborg Vatne, Guro Helén Rogge, Frederik Sebastian Björnsdottir, Valgerdur Lensjø, Kristian Fyhn, Marianne . An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice. Nature Communications. 2023, 14(1), 608 | |
dc.identifier.uri | http://hdl.handle.net/10852/109644 | |
dc.description.abstract | Abstract Genetically encoded Ca 2+ indicators (GECIs) are widely used to measure neural activity. Here, we explore the use of systemically administered PHP.eB AAVs for brain-wide expression of GECIs and compare the expression properties to intracerebrally injected AAVs in male mice. We show that systemic administration is a promising strategy for imaging neural activity. Next, we establish the use of EE-RR- (soma) and RPL10a (Ribo) soma-targeting peptides with the latest jGCaMP and show that EE-RR-tagged jGCaMP8 gives rise to strong expression but limited soma-targeting. In contrast, Ribo-tagged jGCaMP8 lacks neuropil signal, but the expression rate is reduced. To combat this, we modified the linker region of the Ribo-tag (RiboL1-). RiboL1-jGCaMP8 expresses faster than Ribo-jGCaMP8 but remains too dim for reliable use with systemic virus administration. However, intracerebral injections of the RiboL1-tagged jGCaMP8 constructs provide strong Ca 2+ signals devoid of neuropil contamination, with remarkable labeling density. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice | |
dc.title.alternative | ENEngelskEnglishAn updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice | |
dc.type | Journal article | |
dc.creator.author | Grødem, Sverre | |
dc.creator.author | Nymoen, Ingeborg | |
dc.creator.author | Vatne, Guro Helén | |
dc.creator.author | Rogge, Frederik Sebastian | |
dc.creator.author | Björnsdottir, Valgerdur | |
dc.creator.author | Lensjø, Kristian | |
dc.creator.author | Fyhn, Marianne | |
cristin.unitcode | 185,15,29,30 | |
cristin.unitname | Seksjon for fysiologi og cellebiologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 2129352 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature Communications&rft.volume=14&rft.spage=608&rft.date=2023 | |
dc.identifier.jtitle | Nature Communications | |
dc.identifier.volume | 14 | |
dc.identifier.issue | 1 | |
dc.identifier.doi | https://doi.org/10.1038/s41467-023-36324-3 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2041-1723 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 608 | |