dc.date.accessioned | 2024-03-05T20:52:55Z | |
dc.date.available | 2024-03-05T20:52:55Z | |
dc.date.created | 2023-06-29T16:41:57Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Kim, Minsang Cheon, Yeongmi Shin, Dongmin Choi, Jieun Nielsen, Josefine Eilsø Jeong, Myeong Seon Nam, Ho Yeon Kim, Sung-Hak Lund, Reidar Jenssen, Håvard Barron, Annelise E. Lee, Seongsoo Seo, Jiwon . Real-Time Monitoring of Multitarget Antimicrobial Mechanisms of Peptoids Using Label-Free Imaging with Optical Diffraction Tomography. Advanced Science. 2023 | |
dc.identifier.uri | http://hdl.handle.net/10852/109192 | |
dc.description.abstract | Abstract Antimicrobial peptides (AMPs) are promising therapeutics in the fight against multidrug‐resistant bacteria. As a mimic of AMPs, peptoids with N ‐substituted glycine backbone have been utilized for antimicrobials with resistance against proteolytic degradation. Antimicrobial peptoids are known to kill bacteria by membrane disruption; however, the nonspecific aggregation of intracellular contents is also suggested as an important bactericidal mechanism. Here,structure‐activity relationship (SAR) of a library of indole side chain‐containing peptoids resulting in peptoid 29 as a hit compound is investigated. Then, quantitative morphological analyses of live bacteria treated with AMPs and peptoid 29 in a label‐free manner using optical diffraction tomography (ODT) are performed. It is unambiguously demonstrated that both membrane disruption and intracellular biomass flocculation are primary mechanisms of bacterial killing by monitoring real‐time morphological changes of bacteria. These multitarget mechanisms and rapid action can be a merit for the discovery of a resistance‐breaking novel antibiotic drug. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Real-Time Monitoring of Multitarget Antimicrobial Mechanisms of Peptoids Using Label-Free Imaging with Optical Diffraction Tomography | |
dc.title.alternative | ENEngelskEnglishReal-Time Monitoring of Multitarget Antimicrobial Mechanisms of Peptoids Using Label-Free Imaging with Optical Diffraction Tomography | |
dc.type | Journal article | |
dc.creator.author | Kim, Minsang | |
dc.creator.author | Cheon, Yeongmi | |
dc.creator.author | Shin, Dongmin | |
dc.creator.author | Choi, Jieun | |
dc.creator.author | Nielsen, Josefine Eilsø | |
dc.creator.author | Jeong, Myeong Seon | |
dc.creator.author | Nam, Ho Yeon | |
dc.creator.author | Kim, Sung-Hak | |
dc.creator.author | Lund, Reidar | |
dc.creator.author | Jenssen, Håvard | |
dc.creator.author | Barron, Annelise E. | |
dc.creator.author | Lee, Seongsoo | |
dc.creator.author | Seo, Jiwon | |
cristin.unitcode | 185,15,12,0 | |
cristin.unitname | Kjemisk institutt | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2159605 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advanced Science&rft.volume=&rft.spage=&rft.date=2023 | |
dc.identifier.jtitle | Advanced Science | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 24 | |
dc.identifier.pagecount | 0 | |
dc.identifier.doi | https://doi.org/10.1002/advs.202302483 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2198-3844 | |
dc.type.version | PublishedVersion | |