dc.date.accessioned | 2024-03-05T20:32:57Z | |
dc.date.available | 2024-03-05T20:32:57Z | |
dc.date.created | 2023-06-22T15:26:09Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Segura Pena, Dario Hovet, Oda Gogoi, Hemanga Dawicki-Mckenna, Jennine Wøien, Stine Malene Hansen Carrer, Manuel Black, Ben E. Cascella, Michele Sekulic, Nikolina . The structural basis of the multi-step allosteric activation of Aurora B kinase. eLIFE. 2023, 12 | |
dc.identifier.uri | http://hdl.handle.net/10852/109187 | |
dc.description.abstract | Aurora B, together with IN-box, the C-terminal part of INCENP, forms an enzymatic complex that ensures faithful cell division. The [Aurora B/IN-box] complex is activated by autophosphorylation in the Aurora B activation loop and in IN-box, but it is not clear how these phosphorylations activate the enzyme. We used a combination of experimental and computational studies to investigate the effects of phosphorylation on the molecular dynamics and structure of [Aurora B/IN-box]. In addition, we generated partially phosphorylated intermediates to analyze the contribution of each phosphorylation independently. We found that the dynamics of Aurora and IN-box are interconnected, and IN-box plays both positive and negative regulatory roles depending on the phosphorylation status of the enzyme complex. Phosphorylation in the activation loop of Aurora B occurs intramolecularly and prepares the enzyme complex for activation, but two phosphorylated sites are synergistically responsible for full enzyme activity. | |
dc.description.abstract | The structural basis of the multi-step allosteric activation of Aurora B kinase | |
dc.language | EN | |
dc.publisher | eLife Sciences Publications Ltd | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | The structural basis of the multi-step allosteric activation of Aurora B kinase | |
dc.title.alternative | ENEngelskEnglishThe structural basis of the multi-step allosteric activation of Aurora B kinase | |
dc.type | Journal article | |
dc.creator.author | Segura Pena, Dario | |
dc.creator.author | Hovet, Oda | |
dc.creator.author | Gogoi, Hemanga | |
dc.creator.author | Dawicki-Mckenna, Jennine | |
dc.creator.author | Wøien, Stine Malene Hansen | |
dc.creator.author | Carrer, Manuel | |
dc.creator.author | Black, Ben E. | |
dc.creator.author | Cascella, Michele | |
dc.creator.author | Sekulic, Nikolina | |
cristin.unitcode | 185,57,19,0 | |
cristin.unitname | Saarela group - Human immune disorders | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 2157239 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eLIFE&rft.volume=12&rft.spage=&rft.date=2023 | |
dc.identifier.jtitle | eLIFE | |
dc.identifier.volume | 12 | |
dc.identifier.pagecount | 0 | |
dc.identifier.doi | https://doi.org/10.7554/eLife.85328 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2050-084X | |
dc.type.version | PublishedVersion | |
cristin.articleid | e85328 | |
dc.relation.project | NFR/325528 | |
dc.relation.project | NFR/262695 | |
dc.relation.project | NR/NN4654K | |
dc.relation.project | NFR/187615 | |