dc.date.accessioned | 2024-02-29T18:10:11Z | |
dc.date.available | 2024-02-29T18:10:11Z | |
dc.date.created | 2023-06-15T13:57:20Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Shekhar, Sudhanshu Brar, Navdeep Kaur Håkansson, Anders P. Petersen, Fernanda Cristina . Treatment of Mouse Infants with Amoxicillin, but Not the Human Milk-Derived Antimicrobial HAMLET, Impairs Lung Th17 Responses. Antibiotics. 2023, 12(2) | |
dc.identifier.uri | http://hdl.handle.net/10852/108817 | |
dc.description.abstract | Emerging evidence suggests differential effects of therapeutic antibiotics on infant T cell responses to pathogens. In this study, we explored the impact of the treatment of mouse infants with amoxicillin and the human milk-derived antimicrobial HAMLET (human alpha-lactalbumin made lethal to tumor cells) on T cell responses to Streptococcus pneumoniae. Lung cells and splenocytes were isolated from the infant mice subjected to intranasal administration of amoxicillin, HAMLET, or a combination of HAMLET and amoxicillin, and cultured with S. pneumoniae to measure T cell responses. After in-vitro stimulation with S. pneumoniae, lung cells from amoxicillin- or amoxicillin plus HAMLET-treated mice produced lower levels of Th17 (IL-17A), but not Th1 (IFN-γ), cytokine than mice receiving HAMLET or PBS. IL-17A/IFN-γ cytokine levels produced by the stimulated splenocytes, on the other hand, revealed no significant difference among treatment groups. Further analysis of T cell cytokine profiles by flow cytometry showed that lung CD4+, but not CD8+, T cells from amoxicillin- or HAMLET plus amoxicillin-treated mice expressed decreased levels of IL-17A compared to those from HAMLET-exposed or control mice. Collectively, these results indicate that exposure of infant mice to amoxicillin, but not HAMLET, may suppress lung Th17 responses to S. pneumoniae. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Treatment of Mouse Infants with Amoxicillin, but Not the Human Milk-Derived Antimicrobial HAMLET, Impairs Lung Th17 Responses | |
dc.title.alternative | ENEngelskEnglishTreatment of Mouse Infants with Amoxicillin, but Not the Human Milk-Derived Antimicrobial HAMLET, Impairs Lung Th17 Responses | |
dc.type | Journal article | |
dc.creator.author | Shekhar, Sudhanshu | |
dc.creator.author | Brar, Navdeep Kaur | |
dc.creator.author | Håkansson, Anders P. | |
dc.creator.author | Petersen, Fernanda Cristina | |
cristin.unitcode | 185,16,15,0 | |
cristin.unitname | Institutt for oral biologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2154912 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Antibiotics&rft.volume=12&rft.spage=&rft.date=2023 | |
dc.identifier.jtitle | Antibiotics | |
dc.identifier.volume | 12 | |
dc.identifier.issue | 2 | |
dc.identifier.pagecount | 0 | |
dc.identifier.doi | https://doi.org/10.3390/antibiotics12020423 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2079-6382 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 423 | |
dc.relation.project | NFR/273833 | |
dc.relation.project | THON/001 | |