dc.date.accessioned | 2024-02-12T18:16:59Z | |
dc.date.available | 2024-02-12T18:16:59Z | |
dc.date.created | 2023-09-27T15:46:03Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Ellefsen, Christiane Færestrand Lindstad, Linda Klau, Leesa Jane Aachmann, Finn Lillelund Hiorth, Marianne Samuelsen, Anne Berit C. . Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies. Carbohydrate Polymers. 2023, 322, 1-10 | |
dc.identifier.uri | http://hdl.handle.net/10852/107941 | |
dc.description.abstract | Fungal β-glucans have received a lot of interest due to their proinflammatory activity towards cells of the innate immune system. Although commonly described as (1➔3)-β-glucans with varying degree of (1➔6)-branching, the fungal β-glucans constitute a diverse polysaccharide class. In this study, the alkali-soluble β-glucans from the edible mushroom Pleurotus eryngii were extracted and characterized by GC, GC–MS and 2D NMR analyses. The extracts contain several structurally different polysaccharides, including a (1➔3)-β-d-glucan with single glucose units attached at O-6, and a (1➔6)-β-d-glucan, possibly branched at O-3. The immunomodulatory activities of the P. eryngii extracts were assessed by investigating their ability to bind to the receptor dectin-1, and their ability to induce production of the proinflammatory cytokines TNF-α, IL-6 and IL-1β in LPS-differentiated THP-1 cells. Although the samples were able to bind to the dectin-1a receptor, they did not induce production of significant levels of cytokines in the THP-1 cells. Positive controls of yeast-derived (1➔3)-β-d-glucans with branches at O-6 induced cytokine production in the cells. Thus, it appears that the P. eryngii β-glucans are unable to induce production of proinflammatory cytokines in LPS-differentiated THP-1 cells, despite being able to activate the human dectin-1a receptor. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Investigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies | |
dc.title.alternative | ENEngelskEnglishInvestigation of the structural and immunomodulatory properties of alkali-soluble β-glucans from Pleurotus eryngii fruiting bodies | |
dc.type | Journal article | |
dc.creator.author | Ellefsen, Christiane Færestrand | |
dc.creator.author | Lindstad, Linda | |
dc.creator.author | Klau, Leesa Jane | |
dc.creator.author | Aachmann, Finn Lillelund | |
dc.creator.author | Hiorth, Marianne | |
dc.creator.author | Samuelsen, Anne Berit C. | |
cristin.unitcode | 185,15,23,0 | |
cristin.unitname | Farmasøytisk institutt | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2179559 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Carbohydrate Polymers&rft.volume=322&rft.spage=1&rft.date=2023 | |
dc.identifier.jtitle | Carbohydrate Polymers | |
dc.identifier.volume | 322 | |
dc.identifier.doi | https://doi.org/10.1016/j.carbpol.2023.121367 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0144-8617 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 121367 | |
dc.relation.project | NFR/294946 | |
dc.relation.project | NFR/226244 | |