dc.date.accessioned | 2024-01-18T17:53:02Z | |
dc.date.available | 2024-01-18T17:53:02Z | |
dc.date.created | 2023-10-10T13:32:27Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Olsen, Christine Wang, Chencheng Aizenshtadt, Aleksandra Abadpour, Shadab Lundanes, Elsa Skottvoll, Frøydis Sved Golovin, Alexey Busek, Mathias Krauss, Stefan Johannes Karl Scholz, Hanne Wilson, Steven Ray Haakon . Simultaneous LC–MS determination of glucose regulatory peptides secreted by stem cell–derived islet organoids. Electrophoresis. 2023, 44(21-22), 1682-1697 | |
dc.identifier.uri | http://hdl.handle.net/10852/106987 | |
dc.description.abstract | Abstract For studying stem cell–derived islet organoids (SC‐islets) in an organ‐on‐chip (OoC) platform, we have developed a reversed‐phase liquid chromatography–tandem mass spectrometry (RPLC–MS/MS) method allowing for simultaneous determination of insulin, somatostatin‐14, and glucagon, with improved matrix robustness compared to earlier methodology. Combining phenyl/hexyl‐C18 separations using 2.1 mm inner diameter LC columns and triple quadrupole mass spectrometry, identification and quantification were secured with negligible variance in retention time and quantifier/qualifier ratios, negligible levels of carryover (<2%), and sufficient precision (±10% RSD) and accuracy (±15% relative error) with and without use of an internal standard. The obtained lower limits of quantification were 0.2 µg/L for human insulin, 0.1 µg/L for somatostatin‐14, and 0.05 µg/L for glucagon. The here‐developed RPLC–MS/MS method showed that the SC‐islets have an insulin response dependent on glucose concentration, and the SC‐islets produce and release somatostatin‐14 and glucagon. The RPLC–MS/MS method for these peptide hormones was compatible with an unfiltered offline sample collection from SC‐islets cultivated on a pumpless, recirculating OoC (rOoC) platform. The SC‐islets background secretion of insulin was not significantly different on the rOoC device compared to a standard cell culture well‐plate. Taken together, RPLC–MS/MS method is well suited for multi‐hormone measurements of SC‐islets on an OoC platform. | |
dc.language | EN | |
dc.publisher | Wiley-Liss Inc. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Simultaneous LC–MS determination of glucose regulatory peptides secreted by stem cell–derived islet organoids | |
dc.title.alternative | ENEngelskEnglishSimultaneous LC–MS determination of glucose regulatory peptides secreted by stem cell–derived islet organoids | |
dc.type | Journal article | |
dc.creator.author | Olsen, Christine | |
dc.creator.author | Wang, Chencheng | |
dc.creator.author | Aizenshtadt, Aleksandra | |
dc.creator.author | Abadpour, Shadab | |
dc.creator.author | Lundanes, Elsa | |
dc.creator.author | Skottvoll, Frøydis Sved | |
dc.creator.author | Golovin, Alexey | |
dc.creator.author | Busek, Mathias | |
dc.creator.author | Krauss, Stefan Johannes Karl | |
dc.creator.author | Scholz, Hanne | |
dc.creator.author | Wilson, Steven Ray Haakon | |
cristin.unitcode | 185,15,12,0 | |
cristin.unitname | Kjemisk institutt | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2183371 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Electrophoresis&rft.volume=44&rft.spage=1682&rft.date=2023 | |
dc.identifier.jtitle | Electrophoresis | |
dc.identifier.volume | 44 | |
dc.identifier.issue | 21-22 | |
dc.identifier.startpage | 1682 | |
dc.identifier.endpage | 1697 | |
dc.identifier.doi | https://doi.org/10.1002/elps.202300095 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0173-0835 | |
dc.type.version | PublishedVersion | |