dc.date.accessioned | 2023-12-19T16:22:04Z | |
dc.date.available | 2023-12-19T16:22:04Z | |
dc.date.created | 2023-12-14T14:13:22Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Butt, Hussain Shakeel Ulriksen, Emilie Steinbakk Rise, Frode Wangensteen, Helle Duus, Jens Øllgaard Inngjerdingen, Marit Inngjerdingen, Kari Tvete . Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L.. Carbohydrate Polymers. 2024 | |
dc.identifier.uri | http://hdl.handle.net/10852/106478 | |
dc.description.abstract | Daphne mezereum L., an important medicinal plant in Scandinavian folk medicine, was used to treat ailments such as diarrhea, swelling and stomach pain. A range of natural compounds have been isolated, but little attention has been given to the polysaccharides in this plant. Previous work in our group have shown that a polysaccharide enriched fraction from the bark of D. mezereum exhibited pro-inflammatory effects. To pursue this further, the aim of the present work was to isolate and characterize these polysaccharides. From the ethanol-precipitate of a water extract, one neutral (DMP-NF) and one acidic (DMP-AF) fraction was isolated by anion-exchange chromatography. GC, GC–MS and 1D- and 2D-NMR were used to characterize the polysaccharide structures. DMP-NF appeared to be a mixture of arabinan, arabinogalactan and hemicelluloses such as xyloglucan, mannan and xylan. DMP-AF contained a pectic polysaccharide mainly consisting of an unusually long homogalacturonan backbone. Enzymatic treatment by pectinase of DMP-AF yielded DMP-ED, which contained a rhamnogalacturonan-I backbone with arabinan, galactan and arabinogalactan side chains. Both DMP-NF and DMP-ED induced IFN-γ and TNF-α secretion in peripheral blood mononuclear cells (PBMCs), DMP-ED being the most potent fraction. DMP-AF was less active, which might be due to a less sterically available rhamnogalacturonan-I domain. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L. | |
dc.title.alternative | ENEngelskEnglishStructural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L. | |
dc.type | Journal article | |
dc.creator.author | Butt, Hussain Shakeel | |
dc.creator.author | Ulriksen, Emilie Steinbakk | |
dc.creator.author | Rise, Frode | |
dc.creator.author | Wangensteen, Helle | |
dc.creator.author | Duus, Jens Øllgaard | |
dc.creator.author | Inngjerdingen, Marit | |
dc.creator.author | Inngjerdingen, Kari Tvete | |
cristin.unitcode | 185,15,23,20 | |
cristin.unitname | Seksjon for farmasøytisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2213697 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Carbohydrate Polymers&rft.volume=&rft.spage=&rft.date=2024 | |
dc.identifier.jtitle | Carbohydrate Polymers | |
dc.identifier.volume | 324 | |
dc.identifier.doi | https://doi.org/10.1016/j.carbpol.2023.121554 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0144-8617 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 121554 | |