dc.date.accessioned | 2023-10-17T15:33:44Z | |
dc.date.available | 2023-10-17T15:33:44Z | |
dc.date.created | 2023-10-09T16:05:33Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Abadpour, Shadab Niemi, Essi Maria Strid Orrhult, Linnea Hermanns, Carolin de Vries, Rick Parreiras Nogueira, Liebert Haugen, Håvard Jostein Josefsen, Dag Krauss, Stefan Johannes Karl Gatenholm, Paul van Apeldoorn, Aart Scholz, Hanne . Adipose-derived StromalCells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold. Advanced Healthcare Materials. 2023 | |
dc.identifier.uri | http://hdl.handle.net/10852/105577 | |
dc.description.abstract | Intra-portal islet transplantation is currently the only clinically approved beta cell replacement therapy, but its outcome is hindered by limited cell survival due to a multifactorial reaction against the allogeneic tissue in liver. Adipose-derived stromal cells (ASCs) can potentially improve the islet micro-environment by their immunomodulatory action. The challenge is to combine both islets and ASCs in a relatively easy and consistent long-term manner in a deliverable scaffold. W e report on manufacturing the Three-dimensional (3D) bioprinted double-layered scaffolds with primary islets and ASCs using a mix of alginate/nano-fibrillated cellulose (NFC) bioink. W e demonstrate the diffusion properties of the bioink and the supportive effect of human ASCs on islet viability, glucose sensing, insulin secretion and reducing the secretion of pro-inflammatory cytokines. Diabetic mice transplanted with islet-ASC scaffolds reached normoglycemia 7 days post transplantation with no significant difference between this group and the group received islets under the kidney capsules. In addition, animals transplanted with islet-ASC scaffolds stayed normoglycemic and showed elevated levels of C-peptide compared to mice transplanted with islet-only scaffolds. Our data presents a functional 3D bioprinted scaffold for islets and ASCs transplanted to the extrahepatic site and suggest a possible role of ASCs on improving the islet micro-environment. | |
dc.language | EN | |
dc.rights | Attribution-NonCommercial 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.title | Adipose-derived StromalCells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold | |
dc.title.alternative | ENEngelskEnglishAdipose-derived StromalCells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold | |
dc.type | Journal article | |
dc.creator.author | Abadpour, Shadab | |
dc.creator.author | Niemi, Essi Maria | |
dc.creator.author | Strid Orrhult, Linnea | |
dc.creator.author | Hermanns, Carolin | |
dc.creator.author | de Vries, Rick | |
dc.creator.author | Parreiras Nogueira, Liebert | |
dc.creator.author | Haugen, Håvard Jostein | |
dc.creator.author | Josefsen, Dag | |
dc.creator.author | Krauss, Stefan Johannes Karl | |
dc.creator.author | Gatenholm, Paul | |
dc.creator.author | van Apeldoorn, Aart | |
dc.creator.author | Scholz, Hanne | |
cristin.unitcode | 185,51,20,10 | |
cristin.unitname | SFF - Hybrid Technology Hub | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2183026 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advanced Healthcare Materials&rft.volume=&rft.spage=&rft.date=2023 | |
dc.identifier.jtitle | Advanced Healthcare Materials | |
dc.identifier.doi | https://doi.org/10.1002/adhm.202300640 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2192-2640 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 2300640 | |
dc.relation.project | NFR/317790 | |
dc.relation.project | NFR/262613 | |
dc.relation.project | HSØ/2019027 | |