dc.date.accessioned | 2023-08-11T17:03:00Z | |
dc.date.available | 2023-08-11T17:03:00Z | |
dc.date.created | 2023-03-15T15:42:48Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Kommedal, Eirik Angeltveit, Camilla Fløien Klau, Leesa Jane Ayuso-Fernandez, Ivan Arstad, Bjørnar Antonsen, Simen Stenstrøm, Yngve Ekeberg, Dag Gírio, Francisco Carvalheiro, Florbela Horn, Svein Jarle Aachmann, Finn Lillelund Eijsink, Vincent . Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases. Nature Communications. 2023, 14(1) | |
dc.identifier.uri | http://hdl.handle.net/10852/103176 | |
dc.description.abstract | Abstract Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline polysaccharides such as cellulose and are crucial for the conversion of plant biomass in Nature and in industrial applications. Sunlight promotes microbial conversion of plant litter; this effect has been attributed to photochemical degradation of lignin, a major redox-active component of secondary plant cell walls that limits enzyme access to the cell wall carbohydrates. Here, we show that exposing lignin to visible light facilitates cellulose solubilization by promoting formation of H 2 O 2 that fuels LPMO catalysis. Light-driven H 2 O 2 formation is accompanied by oxidation of ring-conjugated olefins in the lignin, while LPMO-catalyzed oxidation of phenolic hydroxyls leads to the required priming reduction of the enzyme. The discovery that light-driven abiotic reactions in Nature can fuel H 2 O 2 -dependent redox enzymes involved in deconstructing lignocellulose may offer opportunities for bioprocessing and provides an enzymatic explanation for the known effect of visible light on biomass conversion. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases | |
dc.title.alternative | ENEngelskEnglishVisible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases | |
dc.type | Journal article | |
dc.creator.author | Kommedal, Eirik | |
dc.creator.author | Angeltveit, Camilla Fløien | |
dc.creator.author | Klau, Leesa Jane | |
dc.creator.author | Ayuso-Fernandez, Ivan | |
dc.creator.author | Arstad, Bjørnar | |
dc.creator.author | Antonsen, Simen | |
dc.creator.author | Stenstrøm, Yngve | |
dc.creator.author | Ekeberg, Dag | |
dc.creator.author | Gírio, Francisco | |
dc.creator.author | Carvalheiro, Florbela | |
dc.creator.author | Horn, Svein Jarle | |
dc.creator.author | Aachmann, Finn Lillelund | |
dc.creator.author | Eijsink, Vincent | |
cristin.unitcode | 185,15,23,30 | |
cristin.unitname | Seksjon for farmakologi og farmasøytisk | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 2134206 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature Communications&rft.volume=14&rft.spage=&rft.date=2023 | |
dc.identifier.jtitle | Nature Communications | |
dc.identifier.volume | 14 | |
dc.identifier.issue | 1 | |
dc.identifier.doi | https://doi.org/10.1038/s41467-023-36660-4 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2041-1723 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 1063 | |
dc.relation.project | NFR/269408 | |
dc.relation.project | NFR/268002 | |
dc.relation.project | EC/HEU/ERC-856446 | |
dc.relation.project | NFR/315385 | |
dc.relation.project | NFR/262853 | |
dc.relation.project | NFR/257622 | |