dc.date.accessioned | 2023-03-18T17:42:59Z | |
dc.date.available | 2023-03-18T17:42:59Z | |
dc.date.created | 2022-12-27T12:12:48Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Kogler, Stian Aizenshtadt, Aleksandra Harrison, Sean Skottvoll, Frøydis Sved Berg, Henriette Engen Abadpour, Shadab Scholz, Hanne Sullivan, Gareth John Thiede, Bernd Lundanes, Elsa Bogen, Inger Lise Krauss, Stefan Johannes Karl Røberg-Larsen, Hanne Wilson, Steven Ray Haakon . "Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry. Analytical Chemistry. 2022, 94(50), 17677-17684 | |
dc.identifier.uri | http://hdl.handle.net/10852/101658 | |
dc.description.abstract | Organoids, i.e., laboratory-grown organ models developed from stem cells, are emerging tools for studying organ physiology, disease modeling, and drug development. On-line analysis of organoids with mass spectrometry would provide analytical versatility and automation. To achieve these features with robust hardware, we have loaded liquid chromatography column housings with induced pluripotent stem cell (iPSC) derived liver organoids and coupled the “organ-in-a-column” units on-line with liquid chromatography-mass spectrometry (LC-MS). Liver organoids were coloaded with glass beads to achieve an even distribution of organoids throughout the column while preventing clogging. The liver organoids were interrogated “on column” with heroin, followed by on-line monitoring of the drug’s phase 1 metabolism. Enzymatic metabolism of heroin produced in the “organ-in-a-column” units was detected and monitored using a triple quadrupole MS instrument, serving as a proof-of-concept for on-line coupling of liver organoids and mass spectrometry. Taken together, the technology allows direct integration of liver organoids with LC-MS, allowing selective and automated tracking of drug metabolism over time. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | "Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry | |
dc.title.alternative | ENEngelskEnglish"Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry | |
dc.type | Journal article | |
dc.creator.author | Kogler, Stian | |
dc.creator.author | Aizenshtadt, Aleksandra | |
dc.creator.author | Harrison, Sean | |
dc.creator.author | Skottvoll, Frøydis Sved | |
dc.creator.author | Berg, Henriette Engen | |
dc.creator.author | Abadpour, Shadab | |
dc.creator.author | Scholz, Hanne | |
dc.creator.author | Sullivan, Gareth John | |
dc.creator.author | Thiede, Bernd | |
dc.creator.author | Lundanes, Elsa | |
dc.creator.author | Bogen, Inger Lise | |
dc.creator.author | Krauss, Stefan Johannes Karl | |
dc.creator.author | Røberg-Larsen, Hanne | |
dc.creator.author | Wilson, Steven Ray Haakon | |
cristin.unitcode | 185,15,12,63 | |
cristin.unitname | Seksjon for kjemisk livsvitenskap - biomolekyler, bio-inspirerte materialer og bioanalytisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 2097526 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical Chemistry&rft.volume=94&rft.spage=17677&rft.date=2022 | |
dc.identifier.jtitle | Analytical Chemistry | |
dc.identifier.volume | 94 | |
dc.identifier.issue | 50 | |
dc.identifier.startpage | 17677 | |
dc.identifier.endpage | 17684 | |
dc.identifier.doi | https://doi.org/10.1021/acs.analchem.2c04530 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0003-2700 | |
dc.type.version | PublishedVersion | |