Drivers of copper sensitivity in copepods: A meta-analysis of LC50s

Abstract

Copper is both an essential trace element and a potent pesticide. The use of copper as an antifoulant has increased in the last decades in line with the expanding aquaculture and shipping industries. In aquatic environments, it also affects non-target taxa. One of which are copepods, which constitute the central link in the marine food web. Despite their ecological importance, there are no systematic reviews of the lethal concentration range and drivers of copper toxicity in this taxon. Here, we combined literature data from 31 peer-reviewed articles recording the Lethal Concentration 50 (LC50) for copper in copepods and the experiments' respective environmental, developmental, and taxonomic parameters. The LC50 is a traditional endpoint for toxicity testing used in standardized toxicity testing and many ecological studies. In total, we were able to extract 166 LC50 entries. The variability in the metadata allowed for a general analysis of the drivers of copper sensitivity in copepods. Using a generalized additive modeling approach, we find that temperature increases copper toxicity when above approximately 25 ℃. Counter to our expectations; salinity does not influence copper sensitivity across copepod species. Unsurprisingly, nauplii are more susceptible to copper exposure than adult copepods, and benthos-associated harpacticoids are less sensitive to copper than pelagic calanoids. Our final model can predict sensible specific-specific copper concentrations for future experiments, thus giving an informed analytical approach to range testing in future dose-response experiments. Our model can also potentially improve ecological risk assessment by accounting for environmental differences. The approach can be applied to other toxicants and taxa, which may reveal underlying patterns otherwise obscured by taxonomic and experimental variability.