dc.date.accessioned | 2023-03-03T18:03:00Z | |
dc.date.available | 2023-03-03T18:03:00Z | |
dc.date.created | 2023-01-11T16:50:32Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Busek, Mathias Aizenshtadt, Aleksandra Koch, Timo Frank, Anna Delon, Ludivine Sylvie Claude Amirola Martinez, Mikel Golovin, Alexey Dumas, Clotilde Stokowiec, Justyna Gruenzner, Stefan Melum, Espen Krauss, Stefan Johannes Karl . Pump-less, recirculating organ-on-a-chip (rOoC) platform. Lab on a Chip. 2022 | |
dc.identifier.uri | http://hdl.handle.net/10852/100653 | |
dc.description.abstract | We developed a novel, pump-less directional flow recirculating organ-on-a-chip (rOoC) platform that creates controlled unidirectional gravity-driven flow by a combination of a 3D-tilting system and an optimized microfluidic layout. The rOoC platform was assembled utilizing a layer-to-layer fabrication technology based on thermoplastic materials. It features two organoid compartments supported by two independent perfusion channels and separated by a hydrogel barrier. We developed a computational model to predict wall shear stress values and then measured the flow rate in the microfluidic channels with micro-Particle-Image-Velocimetry (μPIV). The suitability of the rOoC for functional culture of endothelial cells was tested using HUVECs seeded in the perfusion channels. HUVECs aligned in response to the directional flow, formed a barrier and were able to sprout into the organoid compartments. Next, we demonstrated the viability of human stem-cell derived liver organoids in the organoid compartments. Finally, we show the possibility to circulate immune cells in the microfluidic channels that retain viability without being trapped or activated. The rOoC platform allows growing and connecting of two or more tissue or organ representations on-chip with the possibility of applying gradients, endothelial barriers, microvasculature and circulating cells independent of external tubing and support systems. | |
dc.language | EN | |
dc.rights | Attribution 3.0 Unported | |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | |
dc.title | Pump-less, recirculating organ-on-a-chip (rOoC) platform | |
dc.title.alternative | ENEngelskEnglishPump-less, recirculating organ-on-a-chip (rOoC) platform | |
dc.type | Journal article | |
dc.creator.author | Busek, Mathias | |
dc.creator.author | Aizenshtadt, Aleksandra | |
dc.creator.author | Koch, Timo | |
dc.creator.author | Frank, Anna | |
dc.creator.author | Delon, Ludivine Sylvie Claude | |
dc.creator.author | Amirola Martinez, Mikel | |
dc.creator.author | Golovin, Alexey | |
dc.creator.author | Dumas, Clotilde | |
dc.creator.author | Stokowiec, Justyna | |
dc.creator.author | Gruenzner, Stefan | |
dc.creator.author | Melum, Espen | |
dc.creator.author | Krauss, Stefan Johannes Karl | |
cristin.unitcode | 185,51,20,10 | |
cristin.unitname | SFF - Hybrid Technology Hub | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2105263 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Lab on a Chip&rft.volume=&rft.spage=&rft.date=2022 | |
dc.identifier.jtitle | Lab on a Chip | |
dc.identifier.volume | 23 | |
dc.identifier.issue | 4 | |
dc.identifier.startpage | 591 | |
dc.identifier.endpage | 608 | |
dc.identifier.pagecount | 18 | |
dc.identifier.doi | https://doi.org/10.1039/D2LC00919F | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1473-0197 | |
dc.type.version | PublishedVersion | |
dc.relation.project | NFR/262613 | |
dc.relation.project | NFR/315399 | |
dc.relation.project | NFR/329001 | |
dc.relation.project | HSØ/30629 | |